Characterization of Halobacterium halobium mutants defective in taxis
نویسندگان
چکیده
منابع مشابه
Bacterio-opsin mutants of Halobacterium halobium.
The bacterio-opsin (bop) gene of Halobacterium halobium R1 has been cloned with about 40 kilobases of flanking genomic sequence. The 40-kilobase segment is derived from the (G+C)-rich fraction of the chromosome and is not homologous to the major (pHH1) or minor endogenous covalently closed circular DNA species of H. halobium. A 5.1-kilobase Pst I fragment containing the bop gene was subcloned i...
متن کاملGenetic variability in Halobacterium halobium.
Halobacterium halobium exhibits an extraordinary degree of spontaneous variability. Mutants which are defective in the formation of gas vacuoles (vac) arise at a frequency of 10(-2). Other easily detectable phenotypes, like the synthesis of bacterioruberin (Rub) or the synthesis of retinal (Ret) and bacterio-opsin (Ops), the two components which form the purple membrane (Pum) of H. halobium, ar...
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Stimulation of Halobacterium halobium through its sensory photosystems, PS 370 and PS 565, leads either to a prolonged or to a shortened interval between two reversals of the swimming direction of the cell, the attractant or repellent response. Stimuli are integrated to yield the same response regardless through which photosystem they are given. Simultaneously elicited attractant and repellent ...
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INTRODUCTION .............................................................. 682 PURPLE MEMBRANE ....................................................... 683 BACTEBRIORHODOPSIN: A LIGHT-DRIVEN PUMP FOR PROTONS ..... ..... 686 SECONDARY GRADIENTS: TRANSMEMBRANE MOVEMENTS OF Na+, K', AND Cl................................................................... 689 AMINO ACID TRANSPORT ............... ....
متن کاملbop gene cluster expression in bacteriorhodopsin-overproducing mutants of Halobacterium halobium.
mRNA levels from the bop (bacterio-opsin), brp (bacterio-opsin-related protein), and bat (bacterio-opsin activator) genes in wild-type Halobacterium halobium and two bacteriorhodopsin-overproducing mutants (ET1001 and II-7) were quantitated under conditions in which oxygen levels were steadily depleted and then cultures were either kept in the dark or exposed to light. All three strains showed ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1990
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.172.5.2328-2335.1990